Unphosphorylated IGF1R-kin

2.5

V/E (1/min)

V/E (1/min)

2.0 1.5 1.0 0.5 0.0

0

250

500

750

Phosphorylated IGF1R-kin

100 75 50 25 0

1000

0

250

Unphosphorylated IGF1R-jmk

2

1

0

0

250

500

750

0

100

300

400

Phosphorylated IGF1R-icd

500

400

0.5

0

250

500

750

200 100

0

250

500

750

1000

Peptide (μM)

Unphosphorylated IGF1R-fl

35

300

0

1000

ATP (μM)

Phosphorylated IGF1R-fl

700

V/E (1/min)

30 25 20 15 10 5

600 500 400 300 200 100

0

250

500

750

0

1000

0

100

ATP (μM) Unphosphorylated IGF1R-fl + IGF1

60

200

300

400

500

Peptide (μM)

Phosphorylated IGF1R-fl + IGF1 400

50

V/E (1/min)

V/E (1/min)

200

Unphosphorylated IGF1R-icd V/E (1/min)

V/E (1/min)

100

ATP (μM)

0.0

V/E (1/min)

200

ATP (μM)

1.0

40 30 20 10 0

1000

300

0

1000

1.5

0

750

Phosphorylated IGF1R-jmk

400

V/E (1/min)

V/E (1/min)

3

500

ATP (μM)

ATP (μM)

300 200 100 0

0

100

200

300

400

Peptide (μM)

500

0

100

200

300

ATP (μM)

400

500