MP052 Intersex condition and neoplasia in Prochilodus magdalenae (Characiformes: Prochilodontidae) in the middle basin of the Magdalena River, Colombia. E. Hernández , Universidad de Murcia / Toxicologia; M. Tovar-Bohórquez, Universidad del Magdalena / Grupo de Investigación y Desarrollo Tecnológico en Acuicultura; M. Torres-Chaparro, University of Murcia / Lab. Toxicology-Health Sciences Dpt-Faculty of Veterinary; S. Espin, University of Murcia / Health Sciences Department - IMIB Arrixca UM - Faculty of Veternary; A. Rodríguez-Forero, Universidad del Magdalena / Grupo de Investigación y Desarrollo Tecnológico en Acuicultura; J. Hoya, Piscícola San Silvestre S.A. / Grupo de Investigación en Peces Nativos; A. EstradaPosada, ISAGEN S.A.E.S.P.; A. Garcia-Fernandez, University of Murcia / Health Sciences. Intersex condition, understood as the simultaneous presence of partial or complete testicular and ovarian tissues in the same gonad of a single gonochoristic teleost fish, seems to be associated with the exposure to endocrine disrupting chemicals (EDCs), mainly of anthropogenic origin. The aim of this investigation was to describe macroscopic and microscopic testicular alterations, and serum profiles of the sex steroids 17?-estradiol (E2) and 11ketotestosterone (11-KT), in a case of intersexes of two male populations of P. magdalenae from the middle basin of the Magdalena River, one wild and the other captive. The hematoxylin and eosin (H&E) and trichrome histological colorations were used; serum levels of sex hormones were determined using indirect ELISA. The wild fish (n=62) were caught in "El Opón" swamp and "Mal Abrigo" spout; the captive fish (n=54) belonged to Piscícola San Silvestre S.A. Sampling was conducted weekly from July 1, 2016 to January 31, 2017. The sex hormone levels were compared between testicular development stages (rest, early maturation, late maturation, mature-spawned and regression) and no statistically significant differences were found. Intersex condition was found in 16% of individuals (19% of wild and 13% of captive males). Macroscopically, multiple unilateral or bilateral irregular nodulations were identified, compromising the normal anatomical shape of the testicles. The histopathological lesions identified were: cellular death of the epithelium of the spermatic compartments, cellular dysplasia with pleomorphic cytoplasm and nucleus and the presence of multinucleated cells, inflammation foci, fibrosis of the interstitial space and presence of oocytes within the testicular tissue. Significant differences were identified between serum 11-KT (median=100.4 pg/ml) and E2 (median=39.0 pg/ml) levels in captive males (both intersex and non-intersex fish, p=0.03), and for 11-KT concentrations among the two populations (58.9±51.2 pg/ml for wild and 151.7±157.1 pg/ml for captive fish, p=0.001). In contrast, no significant differences were found between mean 11-KT and E2 concentrations in wild fish, neither between mean E2 values of the two populations. The development of intersex and neoplasia in these P. magdalenae male populations could be related to EDCs exposure. Further studies are essential to identify the potential chemicals involved and the ecological risk for this and other vertebrate species, including humans\n

SETAC Latin America 13th Biennial Meeting

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